Institute of Accelerator Analysis Ltd. (IAA) is the first Contract Research Organization in Japan providing Accelerator Mass Spectrometry (AMS) analysis services which is carbon dating and bioanalysis works. IAA has two AMS machines which is 3 MV Pelletron type for carbon dating works and the other is 0.6 MV for bioanalysis works. These AMS machines are maintained by the validated analysis methods using few control compounds. It is confirmed that these AMS systems have reliabilities and sensitivities enough for each objective. The graphitization of samples of bioanalysis is prepared by our own purification lines including the measurement of total carbon content in the sample automatically.
Recently we presented the utilities of AMS analysis in human mass balance and metabolites profiling studies comparing with LSC results obtained from the same samples. Human samples such as plasma, urine and feces were obtained from four healthy volunteers orally administered of 14C-labeled drug A, of which radioactivity was about 3 MBq ( 85 micro Ci ). For AMS measurement, these samples were diluted 100-fold to 10,000-fold with pure-water or blank samples. The results indicated that AMS method had a good correlation with LSC method (eg. plasma: r = 0.998, urine: r = 0.997, feces: r = 0.997), and that the drug recovery in the excreta exceeded 92 %. The metabolite profiles of plasma, urine and feces obtained with HPLC-AMS corresponded to radio-HPLC results measured at much higher radioactivity level.
These results revealed that AMS analysis is useful to measure 14C-concentration in bioanalysis studies at very low radioactivity level
See more of AMS in Low Dose Biosciences Posters
See more of The 10th International Conference on Accelerator Mass Spectrometry (September 5-10, 2005)